Signaling Role of Exogenous Arachidonic Acid in the Invasion of Macrophages by Toxoplasma gondii
- VernacularTitle:外源性花生四烯酸在弓形虫入侵巨噬细胞中的信号作用
- Author:
Biwen PENG
;
Qingling HUANG
;
Jianyin LIN
;
Mingsen JIANG
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii, invasion, macrophage, arachidonic acid, calcium signal
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the signaling role of arachidonic acid in the invasion of RAW264.7 macrophage by Toxoplasma gondii . Methods Rate of infection was calculated by both light microscope and flow cytometer. Fluorescent emission spectra were recorded using a microspectrofluometer for the concentration of cytoplasmic free calcium. Results Calcium concentration in macrophages and rate of infection increased with a higher concentration of exogenous arachidonic acid in a dose-dependent manner. The invasion was dependent on the mobilization of calcium from the extracellular medium and from intracellular stores and followed the influx of calcium into the parasitized cell. Conclusion Arachidonic acid may enhance the rate of infection via calcium transduction pathway.
