EFFECT ON THE EXPERIMENTAL RESULTS OF THE CONTENT OF COMPOSITIONS IN RAPD EXPERIMENT OF SOIL CULTURED BACTERIA
- VernacularTitle:土壤可培养细菌DNA的提取及RAPD条件的优化
- Author:
Junmin LI
- Publication Type:Journal Article
- Keywords:
Soil cultured bacteria, RAPD, Composition
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The total soil cultured bacterial DNA was extracted by improved CTAB -lysosome-protease K method and was analyzed by random amplified polymorphic D NA markers. The effect of concentration of Mg 2+ , dNTP, DNA templates, pri mers, DNA polymorase and bovine serum albumin on experimental results were teste d and the optimal reaction system of RAPD for soil cultured bacteria was determi ned.
