The study on early diagnosis of invasive aspergillosis by the use of ELISA assay and nest PCR
- VernacularTitle:侵袭性烟曲霉菌感染早期诊断的实验研究
- Author:
Jin YU
;
Ruoyu LI
- Publication Type:Journal Article
- Keywords:
Aspergillosis;
Polymerase chain reaction;
Diagnosis
- From:
Chinese Journal of Laboratory Medicine
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new specie-specific nest PCR to diagnose the invasive aspergillosis and to estimate the detection of circulating galactomannan by sandwich ELISA in immunocompromised patients. Methods (1) To develop an animal model of IA and collect the whole blood and tissues from the animal model. To extract the DNA from the whole blood and perform nest PCR of the DNA ;To perform the nest PCR of the clinical blood samples from immunocompromised patients (2)To detect the circulating galactomannan from the immunocompromised patients samples by use of sandwich ELISA. Results (1) In animal model, the positive rate of nest PCR and blood culture were 77.8% and 16.7%;The sensitivity and specificity of nest PCR in animal model with invasive aspergillosis are 77.8% and 100%;(2) The sensitivity and specificity of nest PCR in immunocompromised patient were 100% and 83.3%;The sensitivity and specificity of sandwich ELISA in immunocompromised patient were 55.6% and 88.2%. Conclusions The positive rate of the nest PCR was higher than that of the blood culture in animal model. The nest PCR method has good sensitivity and specificity in both animal model and patients. It will be a promising method in diagnosis of invasive aspergillosis. The ELISA method will be helpful in diagnosis of invasive aspergillosis.
