Effect of Dendritic Cells Loaded with Tumor Antigens on Antitumor Activity of Tumor-Infiltrating Lymphocytes from Hepatocellular Carcinoma in vitro
- VernacularTitle:肿瘤抗原负载的树突状细胞对肝癌肿瘤浸润淋巴细胞体外抗瘤作用的影响
- Author:
Xiaoying WANG
;
Zhiqiang LIANG
;
Yixin ZHANG
;
Weihong SUN
;
Youqi SHI
- Publication Type:Journal Article
- Keywords:
dendritic cell;
tumor-infiltrating lymphocyte;
cytotoxicity;
hepatocellular carcinoma
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of dendritic cells(DCs) loaded with tumor antigens on the proliferation and antitumor activity of tumor-infiltrating lymphocytes(TILs) from hepatocellular carcinoma (HCC) in vitro . Methods:DCs derived from the peripheral blood of patients with HCC were loaded with autologous tumor lysate(Tuly) to generate DC-Tuly and then the DC-Tuly was co-cultured with autologous TILs in low concentration of IL-2. TILs were counted, and the phenotypes of DCs and TILs were assayed by FCM and the cytotoxicity of TILs was determined with LDH method. The concentration of cytokines in TILs culture supernatant such as IFN-? and TNF-? was detected by ELISA . Results: The expression levels of CD1a, CD80, CD83,CD86, CD40, HLA-DR molecules on the DC-Tuly are notably increased( P
