The Therapentic Effects of Ets-1 Antisense Oligoxydeonucleotide On Gastric Carcinoma
- VernacularTitle:ets-1基因的反义寡核苷酸治疗胃癌的研究
- Author:
Yinlu DING
;
Feng ZHAO
;
Xinzhong CHANG
;
Xianqian GONG
;
Zhaoting LI
- Publication Type:Journal Article
- Keywords:
gastric cancinoma;
ets-1;
antisense oligoxydeonucleotide;
neoplasm invasiveness
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the therapeutic effects of ets-1 antisense oligoxydeonucleotide(AsODN) on gastric carcinoma. Methods: Cultured SGC-7901 cells were devided into control group, sense oligoxydeonucleotide(sODN) group and AsODN group. After transfection for 24 h, expression of ets-1mRNA was detected by RT-PCR, growth inhibition was detected by colone formation assay, in vitro invasive ability assay was carried out in Transwell chamber,the animal model of xenotransplanted gastric carcinoma in nude mice was established to detect invasive ability in vivo. Results: ets-1 AsODN could under-regulate the expression of ets-1 mRNA, number of colone formation of AsODN group was significantly lower than the other two groups(24.2?4.8 vs 47.6?8.1 vs 44.3?7.6, P
