Bioluminescent Monitoring the Influence of Cepharanthine on Colon Cancer Growth in Mouse Xenograft Tumor Model
- VernacularTitle:千金藤素对人结肠癌细胞HCT116裸鼠异体移植肿瘤模型肿瘤增殖的影响及机制初探
- Author:
Jianli GAO
;
Tongchuan HE
;
Guiyuan LV
- Publication Type:Journal Article
- Keywords:
colon cancer;
cepharanthine;
bioluminescence imaging;
tumor cellproliferation;
signaling pathways;
reporter genes
- From:
Journal of Zhejiang Chinese Medical University
2013;(9):1055-1059,1072
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the anti-proliferative effects of CEP on HCT116 cells and in mouse xenograft model. [Methods]The in vivo anti-cancer activity of CEP was determined with Xenogen bioluminescence imaging in a xenograft tumor model. The cel-based multiple signaling pathway reporter assays were carried out to determine the effects of CEP on these pathways. [Results] CEP inhibited growth of human cancer cells, the IC 50 was 0.8~11.5 μM. CEP induced cellcycle arrest in S and G2/M phase. CEP also inhibited xenograft tumor growth in athymic nude mice bearing HCT116 cells. The xenograft tumor size was significantly reduced upon the treatment with CEP(10 or 20 mg·kg-1 body weight) for up to 3 weeks. Pathway-spe-cific reporter assays indicated that CEP effectively suppressed the NF-κB and MAPK/ERK signaling pathways. [Conclusions] Our results suggest that the anticancer activity of CEP in colon cancer cells may be mediated through targeting NF-κB and MAPK/ERK signaling pathways.
