Caspase-3 expression on MCA ischemia-reperfusion in rat models
- VernacularTitle:大鼠大脑中动脉缺血/再灌注模型中Caspase-3的表达
- Author:
Yuhui WANG
;
Fuyuan SHAO
;
Chunlin XIA
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia Model Apoptosis Caspase 3
- From:
Journal of Clinical Neurology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of caspase 3 in ischemic brain damage of rats, and further understand the molecular mechanisms of ischemic cerebral vascular disease.Methods Rat models of the left middle cerebral artery (MCA) occlusion/reperfusion were made using a modification of the intraluminal sature method of Longa established by Belayev, infarct zones were confirmed by 2,3,5 triphenyltetrazolium chloride (TTC) staining, and caspase 3 expression on brain sections at the mRNA and active protein level was detected with in situ hybridization and immunohistochemistry technique, respectively.Results After 2 hours of left MCA ischemia followed by 24 hours of reperfusion, obvious infarct in the MCA dominate regions was confirmed by TTC staining; low levels of caspase 3 mRNA, and fewer of its active protein expression was found in normal brains, sham brains and contralateral brains of MCAO rats; both caspase 3 mRNA and activated protein expression in ipsilateral region were increased after 24 hours of recirculation, and even higher levels were detected at 48 hours of reperfusion.Conclusion Apoptotic mechanism might involve in delayed neuronal death after cerebral ischemia, and caspase 3 might play an important role in ischemic neuronal injury.
