Improvement of the current routine method of WBC counting in cerebrospinal fluid
10.3969/j.issn.1671-8348.2013.21.023
- VernacularTitle:脑脊液白细胞显微镜计数法的改良
- Author:
Lichun HUANG
;
Yuzhen CEN
;
Lei ZHENG
;
Te XIONG
;
Yani CHEN
;
Dehua SUN
- Publication Type:Journal Article
- Keywords:
cerebrospinal fluid;
WBC counts;
microscopic count;
glacial acetic acid
- From:
Chongqing Medicine
2013;(21):2503-2504,2507
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the reliability and accuracy of WBC counting in cerebrospinal fluid (CSF) ,this article is stud-ying the improved method of WBC counting in CSF by finding out the optimum percentage of CSF specimen with the most suitable concentration of acetic acid .Methods CSF specimen was mixed with different acetic acid at different ratio respectively .WBC counts were performed in 5 minutes on diluted samples of various concentrations .A series of 20 CSF specimens were analyzed via the proposed assay and conventional method .The average value and coefficient of variation (CV) of WBC count of each sample were c compared and analyzed .Results The optimum percentage of CSF sample was obtained at 60∶40 ratio .In this percentage , the maximal WBC count (189/μL) was obtained compared that of conventional method (161/μL) .Moreover ,the CV of the WBC counts in this percentage (7% ) was also lower than that of the conventional method (18% ) .Conclusion The reliability and accur-ancy of WBC counting in CSF was the optimum percentage of CSF specimen and 5% acetic acid was 60 :40 .It may lead to a more reliable ,accurate and standard way of WBC counts in CSF .
