Research on molecular genetic basis for Jk(a-b-) phenotype
- VernacularTitle:Jk(a-b-)表型分子机理的初步研究
- Author:
Faming ZHU
;
Xianguo XU
;
Xiaozhen HONG
- Publication Type:Journal Article
- Keywords:
Kidd blood group system;
Jk(a-b-)/phenotype;
DNA sequencing;
Jk(a-b-)/molecular basis
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular basis for Jk(a b ) phenotype.Methods Routine serologic testing for phenotype.Genomic DNA covering 4～11 exons and partial introns of JK gene was amplified by ploymerase chain reaction.The PCR products were excised and purified from agarose gels with a kit,then fragments were directly sequenced.Results G mutated to A in the 3'acceptor splice site of intron 5;A to G at 78 site from the 3'end of intron 3;C to T at 84 site from the 5'end of intron 8; A to G at 588 site of exons ( exon 7); G to A at 838 site of exons (exon 9).The splice site mutation (G→A) of intron 5 may cause the skipping of exon 6.Conclusion G to A mutation in the 3'acceptor splice site of intron 5 maybe one of the molecular basis for Jk(a-b-) phenotype
