Markers of oxygen metabolism of neutrophils in patients with coronary heart disease: detection and clinical significance
- VernacularTitle:冠心病患者中性粒细胞氧化代谢指标的检测及意义
- Author:
Yanling LIU
;
Jianmin WU
- Publication Type:Journal Article
- Keywords:
Coronary disease;
Neutrophils;
Reactive oxygen species;
Cell degranulation;
Peroxidase
- From:
Chinese Journal of Laboratory Medicine
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the production of reactive oxygen species and anti-oxidative protein of neutrophils in patients with coronary heart disease, and to evaluate the role of neutrophil activation in the pathogenesis of coronary heart disease. Methods Reactive oxygen species production was measured by chemiluminescence method, the degranulation of neutrophils was detected by the whole cell patch-clamp capacitance technique, the plasma and intracellular levels of myeloperoxidase(MPO) were quantified by photometry.Results The production of superoxide anion in neutrophils was increased in patients with stable or unstable angina more significantly than control group (344.21?64.4,325.65?65.1 vs 271.53?56.2,P
