Establishment and application of immunoradio assay for detecting human soluble IL-6R?
- VernacularTitle:可溶性白细胞介素-6受体结合链免疫放射检测方法的建立及应用
- Author:
Yuhua QIU
;
Huating ZHU
;
Wei XIE
;
Jianzhong PAN
;
Xueguang ZHANG
- Publication Type:Journal Article
- Keywords:
Receptors, interleukin-6;
Immunoradiometric assay;
Tumor mcrrlsers, biological
- From:
Chinese Journal of Laboratory Medicine
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the sensitive,specific,stable and convenient immunoradio assay for detecting human soluble IL-6R?.Methods The hybridoma cell lines were obtained by fusing spleen cells of BALB/c mice that had been immunized with soluble IL-6R? protein to mouse myeloma cells sp2/0. Ascites were used to produce the monoclonal antibodies (mAbs). The mAbs were purified by protein G immunoaffinity method. The mAb SI10 was used as coating antibody, the other mAb H126 recognized different epitope from SI10 was labeled by 125I. Results The immunoradio assay for detecting soluble IL-6R? was set up. It has high stability and accuracy. The detecting limit is 10 ng/ml. The serum concentration of soluble IL-6R? is (81.96 ? 7.23) ng/ml in healthy donors and (237.58?70.96) ng/ml in patients with multiple myeloma. Significant difference was founded between two groups (P
