Variability analysis of human cytomegalovirus
- VernacularTitle:人类巨细胞病毒的变异性分析
- Author:
Fan YANG
;
Zheng ZHANG
- Publication Type:Journal Article
- Keywords:
Human cytomegalovirus;
Variability;
Restriction enzyme
- From:
Chinese Journal of Laboratory Medicine
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Comparing the variability of four genes of HCMV in the population and the therapy process of single patient.Methods Using PCR to amplify the four genes and restriction enzyme to validate four fragments, These four genes are UL55,UL57,UL86 and UL122 whose coding products are glycoprotein B,single chain binding protein,main capsid protein and IE pp86 separately. Then we use AD169 to optimize the conditions of PCR and restriction enzyme. then we use these conditions to detect 200 blood specimens. Results The PCR positive rate of UL55 is 12.5% and restriction positive rate is 10.0%, which has marked difference with other 3 gene segments(P
