Study on the extraction recovery of docetaxel in plasma by different solid-phase cartridges
10.3969/j.issn.1671-8348.2013.22.023
- VernacularTitle:不同填料固相小柱对多烯紫杉醇血浆样品萃取回收率的影响
- Author:
Jie WANG
;
Yu YU
- Publication Type:Journal Article
- Keywords:
solid-phase extraction;
docetaxel;
HPLC
- From:
Chongqing Medicine
2013;(22):2629-2630,2634
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the solid-phase extraction method by comparison of the extraction recovery of docetaxel plas-ma samples.Methods After extracted by different solid-phase cartridges with water,acetonitrile,and different content methanol e-lution,the plasma samples were analyzed on an Phenomenex LUNA C18 column(4.6 mm×250 mm,5μm)with mehhanol:acetoni-trile:water(42∶26∶32,v/v/v)as the mobile phase.From the recovery achieved,the best solid phase cartridge was found.Results This method consists of using 60%-80% methanol for the elution.Among the three kinds of solid-phase being tested,Waters Oasis HLB cartridge was found to be the best one.Conclusion The average extraction recovery of the Waters Oasis HLB cartrid-ges was between 95%-105%,and it can be used in the analysis of docetaxel in plasma samples.
