Different effect of Ceftazidime on the early and mature Pseudomonas aeruginosa biofilm bacteria
- VernacularTitle:头孢他啶对早期和成熟期绿脓假单胞菌生物膜菌作用的差别
- Author:
Haiping ZHANG
;
Bijie HU
;
Huayin LI
;
Xuehua CHEN
;
Zhaoyan ZHOU
;
Li DONG
;
Lixian HE
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Biofilms;
Ceftazidime
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Ceftazidime on the early and mature Pseudomonas aeruginosa biofilm bacteria. Method The early and mature PA biofilm were formed through the chemostat combined Robbins device. Then 64 ?g/ml Ceftazidime was applied to the early and mature PA biofilm bacteria for 24 hours respectively. Scanning electronic microscope(SEM) was used to observe the effect of Ceftazidime on early and mature biofilm. The difference between the viable bacteria in early and mature biofilm was analyzed by statistical method. Result 24 hours after Ceftazidime was added, Pseudomonas aeruginosa in early biofilm was killed on the whole while there were still numbers of bacteria in mature biofilm. Statistical analysis demonstrates a great variance in viable counts. Conclusion Pseudomonas aeruginosa in mature biofilm is more tolerant to antibiotics than that in early biofilm.
