The immunoprecipitation method for the determination of ?-glutamyltransferase complexed to two lipoproteins in serum and its application for the diagnosis of liver malignancies
- VernacularTitle:免疫沉淀法测定血清中与两种脂蛋白结合的?-谷氨酰基转移酶活性及其诊断肝癌的价值
- Author:
Jiaping YU
;
Aihua WANG
;
Pengfei GU
;
Jun CHEN
- Publication Type:Journal Article
- Keywords:
Liver malignancies;
?-Glutamyltransferase;
Low-density-lipoprotein;
Very-low-density-lipoprotein;
Immunoprecipitation
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the immunoprecipitation method to detect the activity of ?-glutamyltransferase (GGT) complexed to low-density-lipoprotein and very-low-density-lipoprotein in serum (LDL-LDL-GGT) and to observe the diagnostic efficiency in discriminating liver malignancies from other hepatopathy diseases.Methods Using apo B antibody as a precipitant to precipitate the parts of GGT which connected to LDL and VLDL. 65 cases of liver malignancies, 53 cases of cirrhosis, 32 of chronic hepatitis and 75 of health people were analysed. Results Apo B antibody can precipitate LDL and VLDL thoroughly with various serum samples. The withinrun CV are 3 6%～8 2% and the betweenrun CV are 5 5%～9 8%. The linearity range is 0～587 U/L. With the cut off value of 10 U/L, the sensitivity for liver malignancies is 86 2%, the specificities for discriminating liver malignancies from cirrhosis and chronic hepatitis is 67 9% and 81 3% respectively. Conclusion It has important significance for the diagnosis of liver malignancies with the immunoprecipitation method for determination of serum LDL-VLDL-GGT activity.
