Effect of Daphnetin on SOD Activity and DNA Synthesis of Plasmodium falciparum in vitro
- VernacularTitle:瑞香素对体外培养恶性疟原虫超氧化物歧化酶活性及DNA合成的影响
- Author:
Lingyun MU
;
Qinmei WANG
;
Yichang NI
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum, daphnetin, superoxide dismutase (SOD), deoxyribonucleic acid (DNA)
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of daphnetin on superoxide dismutase (SOD) activity and DNA synthesis in P. falciparum in vitro. Methods The effect of daphnetin, daphnetin-Fe complex and desferrioxamine B on SOD activity of P. falciparum (P. f) FOCI in vitro was determined with a SOD test-kit. The level of DNA synthesis of P. f synchronized cultured in vitro at various developmental stages after treatment of daphnetin or desferrioxamine B was assayed by fluorescein Hoechest 33258. Results The total SOD activity decreased by 60% after daphnetin treatment while it only decreased by 22% if treated with desferrioxamine B. No effect on SOD activity of P. f treated with daphnetin-Fe complex was observed. The level of DNA synthesis of P. f trophozoites in synchronized in vitro culture was significantly lower than that of the control. Conclusion Daphnetin lowered SOD activity and decreased DNA synthesis of P. f in vitro.
