cDNA Cloning and Sequence Analysis of Major Allergen of Dermatophagoides farinae (Der f 2) in South China
- VernacularTitle:华南地区粉尘螨主要变应原Derf2的cDNA克隆及序列分析
- Author:
Minqi HAO
;
Jun XU
;
Nanshan ZHONG
- Publication Type:Journal Article
- Keywords:
allergen, Dermatophagoides farinae, cDNA
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone and analyze the cDNA of major allergen Der f 2 of Dermatophagoides farinae in south China. Methods cDNA of Der f 2 was cloned by RT-PCR, screened and their sequences were analyzed. Results cDNAof Der f 2 was cloned. The sequence of the cloned Der f 2 was different with that published (D10448) in GenBank, with 87 additional nucleotides inserted into the 62th nucleotide of the original one. According to the original ORF, the deduced amino acids that were located prior or after the inserted 29 amino acid sequence showed no changes. Conclusion The cDNA of Der f 2 was cloned from Dermatophagoides farinae and its sequence showed significant difference with that reported in the GenBank.
