Proliferation and Immunophenotypic Analysis of CD3AK Cells Derived from Lymph Node
- VernacularTitle:淋巴结来源的CD3AK细胞的增殖和表型变化分析
- Author:
Shenglin MA
;
Jianguo FENG
;
Shenhua XU
;
Lirong TANG
;
Yutian LIN
- Publication Type:Journal Article
- Keywords:
CD3 single clone antibody;
flow cytometry;
CD3AK cell
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the propagation and phenotypes changes of killer cell (CD3AK cell activated by CD3 mAb in vitro. Methods: Lymph nodes taken from lung cancer patient is dissociated into single cell suspension by mechanical method and cultured in culture medium added CD3 mAb and a little dose IL-2. We analyze cell immunophenotype by flow cytometry and proliferation by trypan blue exclusion test per 2 days. Results: Immunophenotypic analysis showed that CD3AK expressing CD3, CD8, CD56, CD25 increased, and reached a peak value which is 2.33 times than before culturing in the 8 th day. Conclusion: CD3 mAb added to the culture medium can obviously activate CD3AK cell and stimulate proliferation and keep its killer activity.
