The influence of chlorderazin on the change of L-arginine transport in human erythrocytes induced by oxidized low density lipoprotein
- VernacularTitle:氯丙嗪逆转氧化低密度脂蛋白对人红细胞左旋精氨酸转运的抑制作用
- Author:
Ronghua WANG
;
Baowei ZHANG
;
Hui XIONG
- Publication Type:Journal Article
- Keywords:
Oxidized low density lipoprotein;
Red blood cell;
L-arginine/nitric oxide pathway;
Chlorderazin
- From:
Chinese Journal of Interventional Cardiology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of chlorderazin on the change of L-arginine transport in human erythrocytes induced by oxidized low density lipoprotein. Methods Human erythrocytes were obtained from six healthy donors. The study included three groups: Gr1 (control)? Gr2 (incubated with OX-LDL) and Gr3 (incubated with OX-LDL and chlorderazin, a PLA 2 inhibitor). The 3H-L-arginine transport was measured.Results Compared with Gr1, the Vmax for the total transport of L-arginine in erythrocytes in Gr2 decreased by 33%, and Km value increased. Compared with Gr2, the Vmax for the total transport and the transport via system y+ of L-arginine in erythrocytes in Gr3 increased by 37% and 25% respectively, and Km values decreased for both. But the change of L-arginine transport by system y+L was not statistically significant.Conclusion OX-LDL could inhibit L-arginine transport in human erythrocytes, which could be reversed by chlorderazin, a nonspecific PLA 2 inhibitor.
