Expression of two neuron developmental associated genes induced by hyperphenylalanine with real time quantitative RT-PCR
- VernacularTitle:荧光定量逆转录聚合酶链反应检测高苯丙氨酸对2种神经元发育相关基因表达的影响
- Author:
Huiwen ZHANG
;
Xuefan GU
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Hyperphenylalaninemia;
Gene expression;
Neuron
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression of two neuron developmental associated genes induced by hyperphenylalanine.Methods Primary embryonal rat cerebral cortical neurons were cultured for three day and cells induced by hyperphenylalanine for 12 hours.Real time quantitative RT-PCR was used to determine the influence of hyperphenylalanine on the expression of GAP-43 and Go?1 genes.Results GAP-43 mRNA was upregulated to 2.25 times and Go?1 was downregulated to 3.31 times by hyperphenylalanine.Conlusion Hyperphenylalanine may interfere the normal development of cerebral cortical neurons through influencing the expression of GAP-43 and Go?1 genes.
