Quantitative determination of LP(a)-C by agarose gel electrophoresis and its clinical application
- VernacularTitle:电泳法测定血清脂蛋白(a)胆固醇及其临床应用
- Author:
Xinyou XIE
;
Xiaoyan CHEN
;
Hongjun CHEN
;
Jianan WANG
;
Chunrong FEI
- Publication Type:Journal Article
- Keywords:
Electrophoresis;
Lipoprotein(a);
Cholesterol;
LP(a)-C determination
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate an agarose gel electrophoresis for quantitative determination of Lipoprotein(a) cholesterol and its clinical application.Methods Quantification of LP(a)-C was performed by a new agarose gel electrophoresis method that allows the separation of LP(a) by Hellen REP system and the determination of LP(a)-C by enzymatic staining of cholesterol,The results of electrophoresis method were compared with the ones of INA and ITA. The specimens of 135 health men were assayed by electrophoresis for the reference range.Results The inter and intra CV of electrophoresis method at low, middle and high LP(a) concentration specimens were 4.7%～5.3% and 5.8%～6.4%. The linearity was 0.058～1.55 mmol/L. Interference with bilirubin (
