Detecting a novel CXC chemokine macrophage inflammatory protein-2? mRNA by real-time fluorescent quantitative reverse transcription polymerase chain reaction (TaqMan)
- VernacularTitle:TaqMan实时荧光定量逆转录聚合酶链反应检测新型趋化因子巨噬细胞炎症蛋白-2?mRNA表达水平
- Author:
Jiahui YANG
;
Qian SHEN
- Publication Type:Journal Article
- Keywords:
Fluoroimmunoassay;
Polymerase chain reaction;
Chemotactic factors,macrophage;
RNA,Messenger
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To develop and evaluate a real-time fluorescent quantitative RT-PCR method for the detection of a novel CXC chemokine macrophage inflammatory protein-2?(MIP-2?)mRNA expression based on TaqMan technique.Method The expression of MIP-2? mRNA was detected by TaqMan real time fluorscet quantitative RT-PCR. Results The dynamic range of the assay varied from 102 to 107 copies. The intra- and inter-assay coefficient variation (CV) were less than
