The dynamic changes of CPP32 mRNA in hippocampus following temporary whole cerebral ischemia in rats
- VernacularTitle:短暂性全脑缺血后大鼠海马CPP32 mRNA表达水平的动态变化
- Author:
Fang HUA
;
Xiubin FANG
;
Yan LU
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia Hippocampus CPP32 mRNA
- From:
Journal of Clinical Neurology
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the dynamic changes of CPP32 mRNA in hippocampus following temporary whole cerebral ischemia in rats and to explore the mechanism of neuron apoptosis induced by cerebral ischemia.Methods The model of temporary whole cerebral ischemia in rats were induced by carotid artery negative pressure shunt and the expression of CPP32 mRNA were detected with fluorescence quantify RT PCR.Results Compared with the naive group, the expression of CPP32 mRNA did not increase insignificantly in sham operation group. Compared with sham operation group, the expression of CPP32 mRNA in ischemia group trended to increase at 6 h after ischemia and increased by 53% at 12 h and 223% at 24 h after ischemia. It kept in high level and decreased at 72 h after ischemia.Conclusion The expression of CPP32 mRNA in hippocampus could be induced by temporary whole cerebral ischemia. It began to increase at 6 h, reached its fastigium at 24 h and tended to decrease at 72 h after ischemia. The enhanced gene transcription of CPP32 plays an important role in the neuron apoptosis induced by cerebral ischemia.
