Establishment and clinical application of collagen binding assay for von Willebrand factor
- VernacularTitle:von Willebrand因子胶原结合试验的建立及其临床应用
- Author:
Wenyu SHI
;
Yingchun WANG
;
Xia BAI
;
Deyan LU
;
Changgeng RUAN
- Publication Type:Journal Article
- Keywords:
von Willebrand factor;
Enzyme-linked immunosorbent assay;
Collagen binding assay
- From:
Chinese Journal of Laboratory Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new methA of detecting vWF function. Methas The capability of vWF to bind collagen was evaluated with ELISA. Results The assay′s sensitivity was 0.001 U/ml. Coefficient of variation for inner-batch and inter-batch were 3.34 and 6.70 respectively.The vWF:CBA value of plasma was(90.24?22.87)% in 20 normal subjects. The vWF:CBA value was (31.94?27.36)% in 54 vWD, (35.22?20.02)% in 10 type 1 vWD, (8.74?6.38)% in 10 type 2A vWD and (0.70?0.58)% in 6 type 3 vWD,the values of all four vWD groups were lower than that of normal group( P
