A novel enzymatic method for measuring serum conjugated bilirubin
- VernacularTitle:结合胆红素酶法测定的一种新方法
- Author:
Jianhua ZHAO
;
Yusan WANG
- Publication Type:Journal Article
- Keywords:
Bilirubin;
Jaundice;
Enzymatic method
- From:
Chinese Journal of Laboratory Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new enzymatic method for the determination of serum conjugated bilirubin (CB) by the use of bilirubin oxidase (BOD). Method Only the CB was oxidized by BOD under the conditions of pH 5.5 in the presence of sodium fluoride NaF and N-acetylcysteine (NAC) which could prevent the unconjugated and delta bilirubin oxidized. CB was quantitatively determined from a decrease in the absorbance at 450 nm caused by CB oxidization. Results The intra-assay CVs were 6.52% and 0.33% (n=20) at CB concentrations of 17.65 and 301.49 ?mol/L, respectively. The inter-assay CVs were 9.90% and 2.72% at 31.50 and 184.12 ?mol/L, respectively. The linearity was up to 320 ?mol /L. The optimum concentrations of selective inhibitors were 2.5 mmol/L for NaF and 2.5 mmol/L for NAC. The results of serum CB (Y) determined by this method correlated well with those determined by diazo-dye method (Y=0.839X-7.965, r=0.969). Conclusion The proposed reaction conditions could effectively inhibit the oxidation of unconjugated bilirubin by BOD, and thus increase the specificity of CB assay.
