Determination of Free Thiols in the Chimeric Protein PfCP-2.9 of Plasmodium faldparum
- VernacularTitle:恶性疟原虫融合蛋白PfCP-2.9自由巯基的测定
- Author:
Feng QIAN
;
Weiqing PAN
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum, chimeric protein, reverse phase HPLC, Ellman' reaction, free thiol
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the free thiols in the chimeric protein PfCP-2. 9 of Plasmodium falciparum expressed by Pichia pastoris. Methods Two experiments of reverse phase HPLC and Ellman' s reaction were applied to the PfCP-2.9 for the determination of its free thiols. For RP-HPLC analysis, three kinds of samples were tested: PfCP-2. 9, dithiothreitol-reduced PfCP-2.9 and indoacetic acid-alkylated PfCP-2.9. Results Both experiments showed that there were no any free thiols present in the PfCP-2. 9. Conclution The disulfide bonds between cysteine residues of PfCP-2. 9 were formed completely.
