Expression of lipocalin-type prostaglandin D synthase in glioma
- VernacularTitle:脂质运载蛋白型前列腺素D合酶在脑胶质瘤中的表达研究
- Author:
Bing SU
;
Ming GUAN
;
Weiwei LIU
;
Yuan L
- Publication Type:Journal Article
- Keywords:
Prostaglandin D;
Reverse transcriptase polymerase chain reaction;
Glioma
- From:
Chinese Journal of Laboratory Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level of lipocalin-type prostaglandin D synthase (L-PGDS) mRNA and its protein in glioma.Methods The L-PGDS mRNA was determined by real-time quantitative RT-PCR in 23 glioma and 5 healthy brain specimens. L-PGDS protein was detected by Western blot in cerebrospinal fluid (CSF) and brain tissue specimens.Results In 23 glioma specimens L-PGDS mRNA expression ranges from 1.5?10 3～7.0?10 4 copy/?g RNA, mean value is 1.6?10 4 copy/?g RNA. In 5 normal brain specimens, the mean value is 8.1?10 5 copy/?g RNA, statistic analysis indicates a distinct discrepancy ( P
