Identification of Listeria monocytogenes by multiplux PCR
- VernacularTitle:多重聚合酶链反应鉴别单核细胞增生李斯特菌的研究
- Author:
Xiaofeng ZHANG
;
Aiyun LI
;
Weihuan FANG
;
Lingli JIANG
;
Chunlin KE
- Publication Type:Journal Article
- Keywords:
Listeria monocytogenes;
Genes;
Polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a rapid and specific method for identification of listerial species and differentiation of Listeria monocytogenes。 Method Primers targeting the iap and hly genes specific for listerial species and L monocytogenes were designed。 The dulplex PCR and triplex PCR were compared for their ability to identify and differentiate the listerial species。 Results Both dulplex PCR and triplex PCR are specific to all listerial reference strains. The dulplex-PCR could not identify some of the L. monocytogenes isolates while the triplex PCR did. Conclusion The triplex PCR method could be used not only for identification of listerial species but also for differentiation of L. monocytogenes from the mixed listerial suspensions.
