Establishment of a rapid quantitative method for Bacillus anthracis
- VernacularTitle:一种快速定量检测炭疽杆菌方法的建立
- Author:
Suhong CHEN
;
Minli ZHANG
;
Hang MU
;
Wei GUAN
;
Shengqi WANG
- Publication Type:Journal Article
- Keywords:
Bacillus anthracis;
Polymerase chain reaction;
Molecular probe techniques
- From:
Chinese Journal of Laboratory Medicine
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid specific quantitative assay for Bacillus anthracis detection. Methods According to the principle of complex probe quantitative assay, the primers and quantitative probes targeted at chromatosome DNA rpoB were designed and applied to detect Bacillus anthracis. The influence factor of quantitative PCR were determined. Results The optimal system of this method was aquired: the length of quenching probe is 15mer,the ratio of fluorescent probe to quenching probe is 1/2 and the concentrtion of Mg 2+ is 3 mmol/L.The sensitivity of this assay for Bacillus anthracis is 10 3 copies. It can distinguish Bacillus anthracis from other closely related Bacillus. Conclusion The method can rapidly quantitatively detect the Bacillus anthracis with high sensitivity and specificity, it can be applied to clinical diagnosis.
