Detecting the expression of mcm5 play the role in diagnosising bladder cancer in urine exfoliate cells
- VernacularTitle:检测尿脱落细胞中微小染色体维持蛋白5基因表达对膀胱癌的意义
- Author:
Man ZHANG
;
Cheng AN
;
Yan BAI
;
Xiumin ZHANG
;
Lin HAO
;
Min ZHANG
;
Hualin XU
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Polymerase chain reaction;
MCM protein 5;
Gene expression
- From:
Chinese Journal of Laboratory Medicine
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of MCM5 gene in urine exfoliate cell and bladder cancer tissue in order to research the diagnosis and difference. Methods We collected the samples of urine exfoliate cell and bladder cancer tissue and extracted the total RNA, and then did RT-nest-PCR, immunohistology to check the expressive level of mcm5 gene. Results Sensitivities of that are 93。3% in 30 bladder cancer, 13。3% in other patients and 0% in normal person. The expression of mcm5 between G1?G2 and G3 bladder cancer tissue have very marked statistic significance ( P
