Identification of Taenia saginata by mtCOⅠ in Four Areas of Yunnan and Guizhou Provinces
- VernacularTitle:用mtCO技术测定云南及贵州四地区的牛带绦虫
- Author:
Zhengrong WANG
;
Huaien BAO
- Publication Type:Journal Article
- Keywords:
Taenia saginata, Taenia saginata asiatica, mitochondrial cytochrome C oxidaseⅠ
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the types of Taenia saginata isolated from Dali of Yunnan Province, and from Duyun and Congjiang of Guizhou Province.\ Methods\ Genomic DNA was isolated, and the mitochondrial cytochrome C oxidase subunitⅠ(mtCOⅠ) genes were amplified by polymerase chain reaction(PCR) and analyzed by PHYLIP software package.\ Results \ The mtCOⅠgene sequences of Lanping sample were identical to that of T\^saginata asiatica known in Taiwan, and the samples obtained from Dali and Duyun showed the same mtCOⅠgene sequences, while the sample from Congjiang had the same mtCOⅠgene sequences as T\^saginata . The homology between these two groups of gene sequences was 97\^44%, while the homology of amino acid sequences reached to 99\^16%. The constructed phylogenetic tree revealed that the relationship between T\^saginata asiatica and T\^saginata is closer, both are distant relative to T\^solium and other species of cestodes.\ Conclusion \ The Taenia prevalent in Lanping, Dali and Duyun is identified as T\^saginata asiatica , while that isolated in Congjiang is the typical T\^saginata.
