Cloning and Characterization of Mitochondrial NADH Dehydrogenase Gene of Cysticercus cellulosae
- VernacularTitle:猪囊尾蚴线粒体NADH脱氢酶的基因克隆
- Author:
Li ZHANG
;
Dianwu LIU
- Publication Type:Journal Article
- Keywords:
Cysticercus cellulosae, cDNA library, NADH dehydrogenase
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone and characterize the NADH1 gene of Cysticercus cellulosae. \ Methods\ A \{cDNA\} library was constructed from Cysticercus cellulosae and was immunoscreened by using rabbit anti\|Cysticercus cellulosae \{polyclonal\} antibody. The gene structure and its possible function were analyzed by comparing with sequences available in the GenBank, after the insert of positive clone was subcloned and the nucleotide sequence of the insert was \{determined\} by dideoxynucleotide chain termination method. \ Results and Conclusion \ A cDNA clone (named TS5) with a length of \{1 082\} bp was isolated. The 5′ terminal of cloned gene contained one open reading frame of 1-578 bp encoding 192 amino acid residues of mitochondrial NADH dehydrogenase subunit 1 and the 3′ terminal contained three kinds of tRNA genes.
