Influence of constant magnetic fields on the proliferation of human umbilical arterial vascular smooth muscle cells pretreated with AngⅡ
- VernacularTitle:恒磁场对血管紧张素Ⅱ预处理的人脐动脉血管平滑肌细胞增殖的影响
- Author:
Guoliang JIA
;
Xuyang FENG
;
Rongqing ZHANG
- Publication Type:Journal Article
- Keywords:
Constant magnetic field;
Vascular smooth muscle cells
- From:
Chinese Journal of Interventional Cardiology
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of constant magnetic fields (CMF) on angiotensinⅡ (AngⅡ)-stimulated proliferation of human umbilical arterial vascular smooth muscle cells (VSMC). Methods The experimental proliferation models of cultured human umbilical arterial VSMC stimulated with AngⅡ was establishea. The VSMC were cultured under 1 and 5 mT CMF for 48 hrs. Proliferation of the VSMC was detected by MTT and 3H-TdR incorporation method (A-value and cpm-value), and cell cycle was analyzed by flow cytometry. Results The CMF of 1 and 5 mT may antagonize proliferation of VSMC stimulated with AngⅡ, and hold-back VSMC from static phase (G 0/G 1)to DNA synthetic (S) and mitotic phase (G 2/M). Conclusion The study demonstrates that CMF of 1 and 5 mT can significantly inhibit the human VSMC proliferation.
