Study on Quality Standard of Qingfei Pill

VernacularTitle:清肺丸质量标准研究
Author: Yang LI ; Jian HUANG ; Zhifeng CHEN ; Sijia SHI ; Kechen MAO
Publication Type:Journal Article
Keywords: Qingfei pill; Fructus Termininaliae Immaturus; Radix Sophorae Subprostratae; Gardenoside; TLC; HPLC
From: Chinese Journal of Information on Traditional Chinese Medicine 2013;(7):64-65,66
CountryChina
Language:Chinese
Abstract: Objective To set up the quality standard of TCM preparation Qingfei pill. Methods TLC methods were used to the qualitative identification of Fructus Termininaliae Immaturus and Radix Sophorae Subprostratae in the preparation. The content of Gardenoside, the effective component of Fructus Gardeniae, was determined by HPLC method, using Agilent Eclipse XDB-C18 (150 mm×4.6 mm, 5 μm) and acetonitrile-water (10∶90) as mobile phase with flow rate at 1.0 mL/min. The column temperature was room temperature, and the detection wavelength was 238 nm. Results The TLC methods for Fructus Termininaliae Immaturus and Radix Sophorae Subprostratae identifications were simple and rapid, and showed good repetitiveness. Gardenoside presented a good linear relationship within the range of 0.238-3.808 μg, and the average recovery rate was 97.24%, RSD was 1.60% (n=6). Conclusion These methods showed strong specificity and good repetitiveness, and can be used to control the quality of the product effectively.