Detection of YMDD mutation during lamivudine therapy by real time fluorimetry PCR
- VernacularTitle:实时荧光聚合酶链反应检测乙型肝炎病毒聚合酶基因变异
- Author:
Chuanmiao LIU
;
Xinxin ZHANG
;
Donghua ZHANG
;
Zhimeng LU
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Polymerase chain reaction;
Variation
- From:
Chinese Journal of Laboratory Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Setting up a method to detect YMDD mutation during lamivudine therapy. Methods Two specific fluorescent probes were designed, on the unpaired base can remarkably affect melting temperature when oligonucleotide probe binding to DNA template. 297 patients (354 sera samples) were detected YMDD mutation by real time fluorimetry PCR. Results 156 YMDD mutation were found in 354 sera samples (44.1%), in which the mixed variant species were 34%(53/156). In the same time, nine sera samples wre sequenced. The results of sequencing were fully corresponded to those of fluorimetry PCR. The statistical analysis showed that YMDD variants were obviously correlated with ALT level(P
