Experimental research of sodium phenylacetate on induced differentiation in cerebral glioma cell
- VernacularTitle:苯乙酸钠对脑胶质瘤细胞诱导分化的实验研究
- Author:
Qingjiu HUANG
;
Tianming YANG
;
Yaohua LI
- Publication Type:Journal Article
- Keywords:
Sodium phenylacetate Inducer Tumor differentiation Glioma
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the curative effect of sodium phenylacetate (NaPA) on induced differentiation in human glioma cells P 168 in vitro,and preliminarily probe into its mechanism.Methods MTT assay flow cytometry and cell microscope were used to test the changes of P 168 cells treated by different concentrations of NaPA in vitro, and observed ultrastructureal changes of tumor cells.Results NaPA could inhibite the growth of human glioma,show the inhibition effect of time concentration dependent,the flow cytometric was used to find S phase is 11.99% or 13.17% in the NaPA treated P 168 cells and 20.17% in the NaPA untreated P 168 cells. Electron microscope of NaPA treated P 168 cells demonstrated there were rich mitochondria, rough endoplasmic reticulum and elastic filament,but in NaPA untreated P 168 cells,there were numerous scattered polyribosomes appeared.Conclusion NaPA not only inhibit the growth of P 168 haman glioma cells, but also remarkably induce the differentiation of these cells in vitro.
