Study on Hydrolysis of chitosan by Lysozyme
- VernacularTitle:溶菌酶对壳聚糖降解的研究
- Author:
Sunying ZHOU
;
Ping YU
;
Sheng CHEN
- Publication Type:Journal Article
- Keywords:
chitosan;
degrade;
Lysozyme;
low molecular chitosan
- From:
Chinese Journal of Marine Drugs
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects of reaction temperature, pH, substrate and enzyme concentration, deacetylation degree of chitosan on the hydrolytic velocity of chitosan by Lysozyme were investigated. The results showed that the specificity of catalyst obey the dynamics of michae-lis-Menten. The optimum temperature and pH were 50℃ and 6. 0 respectively, the reaction velocity was reduced while the deacetylation degree of chitosan was raised. The mean molecular weight of low molecular chitosan has been determined by high performance liquid chro-matography method, the mean molecular weight was 8328.
