Full-length nucleotide sequence analysis of the S and M segments in Z5 strain of Hantavirus Z5 strain
10.3969/j.issn.1002-2694.2010.03.006
- VernacularTitle:汉坦病毒Z5株M和S片段全基因序列测定及分析
- Author:
Chan LI
;
Ronghui XIE
;
Hanping ZHU
;
Fang XU
;
Pingping YAO
;
Yinkai CHENG
;
Zhiyong ZHU
- Publication Type:Journal Article
- Keywords:
Hantavirus;
M and S segment;
sequence analysis
- From:
Chinese Journal of Zoonoses
2010;(3):215-217,225
- CountryChina
- Language:Chinese
-
Abstract:
The M and S segment cDNAs of hantavirus Z5 strain was amplified by RT-PCR,and the purified PCR products were cloned into vector pGEM-T and then sequenced.It was demonstrated that the M genome segment of Z5 was found to be 3 616 nucleotides in length with a single open reading frame encoding 1 135 amino acids.And the S genome segment was 1700 nucleotides in length with a single open reading frame encoding 429 amino acids.As demonstrated by the homologous analysis of nucleotides and amino acids,it was showed that the Z5 strain belonged to hantaan viruses HTN type and was the same subtype of the Z10 strain.It is conclouded that difference in nucleotide sequence exists between Z5 strain with other Hantavirus strains but high level of homology in amino acid sequences is still present.
