The research on TEM ?-lactamase genes of Acinetinbacter lwoffi
- VernacularTitle:洛菲不动杆菌TEM型?内酰胺酶基因研究
- Author:
Ping YAO
;
Xiujuan YU
;
Qinglian KONG
- Publication Type:Journal Article
- Keywords:
Acinetobacter;
Escherichia coli;
Gene;
Sequence homology,nucleic acid
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objects To observe the TEM gene characteristic of Acinetinbacter lwoffi strains which were resistant to most kind of antibiotics and to compare their sequences with that of Escherichia coli Methods Two strains of A lwoffi, JN18 and JN70,were isolated from sputum of patients′ who suffered from respiratory infection TEM, SHV, OXA, IMP and CTX M genes were tested by PCR TEM sequences of JN18 and JN70 were detected by ABI automated sequencer and were analysed by DNAStar software to compare the differences with E coli TEM genes that had been published in GenBank Results Detected sequences of A lwoffi JN18 and JN70 strains were 1012 bp and 887 bp, respectively They coded regions of 832 bp and 772 bp for TEM of the two strains that were 98 2% identical In other hand, there were 14 pair bases differently in TEM regions The TEM sequence of JN18 was 98 72% identical to that of E coli TEM on the average, which was over 99% to TEM1D, TEM 70, TEM 76, TEM 77 and TEM 95 of E coli The divergence of TEM genes was 1 26 between A lwoffi JN18 strains and E coli JN70 strain had higher identity (98 93%), which reached 99 5% to TEM1D, TEM1F and TEM84 of E coli As JN18 strain, TEM gene of JN70 had very small divergence (1 06 ) with E coli Conclusion JN18 and JN70 strains of A lwoffi isolated from patients′respiratory tract in Jinan shared most of sequences with E coli TEM76 and 84 However, there were many mutant sites in them
