Activation of nuclear factor-?B in cultured human proximal tubular cell by low density lipoprotein in expression of plasminogen activator inhibitor-1 and tissue plasmin activator
- VernacularTitle:低密度脂蛋白激活人肾小管上皮细胞核转录因子?B与纤溶酶系统表达
- Author:
Limeng CHEN
;
Xuewang LI
;
Jiayou LIN
;
Hang LI
;
Lin DUAN
- Publication Type:Journal Article
- Keywords:
Low density lipoprotein;
Plasminogen activator inhibitor 1;
Tissue plasmin activator;
Nuclear factor ?B
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the activity and mRNA expression of plasminogen autivator inhibitor (PAI 1) and tissture plasmin activator (tPA) stimulated by low density tipoprotein (LDL) on cultured human proximal tubular cell (HKC), which was a cell line of human proximal tubular cell. To show whether LDL can lead to the activation of nuclear factor ?B NF ?B, and weather the effect can be reversed by Lovastatin, a kind of 3 hydroxy 3 methylglutaryl coenzyme, a reductase inhibitors (HRI). Methods Chromogenic substance was used to show the activity of tPA and PAI 1, RT PCR showed the mRNA expression of PAI 1 and tPA. The expression of P65 in nuclear was showed by Laser confocal microscopy. Results LDL could up regulate the activity of PAI 1 , down regulate the activity of tPA, which was decreased from 6.22?0.52 IU/ml to 4.9?0.11 IU/ml (in control vs LDL, P
