Experimental study of co-immunizations of HIV-1 gag-hIL-2 recombinant vaccinia virus and DNA vaccine pIRES-gag-hIL-2
- VernacularTitle:HIV-1中国流行株gag-hIL-2重组痘苗病毒与pIRES-gag-hIL-2核酸疫苗联合免疫的实验研究
- Author:
Lixin WANG
;
Jun LIN
;
Hongwei WANG
- Publication Type:Journal Article
- Keywords:
HIV 1;
gag;
hIL 2;
Vaccinia virus;
DNA vaccine
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain recombinant vaccinia viruses of gag gene,and hIL 2 gene of HIV 1CN subtype B,evaluate the immune effects after immunization together with nucleic acid vaccine for AIDS vaccine development.Methods gag gene,and hIL 2 gene were inserted downstream of the combined promotor of pJ38 vector.Recombinant vaccinia viruses were selected by using plaque assay.Expression products were examined by SDS PAGE and Western blot.Immune response of BALB/c mice immunized was evaluated by lymphocyte transformation test and OD value of serum IgG antibody.Results Recombinant vaccinia viruses vJ38gag IL 2 were obtained with reactinogenicity.Effect of recombinant vaccinia viruses immunized three times was better than that immunized two times.The best result was obtained in the rVV primed and nucleic acid vaccine boosted group.Conclusion Gag protein and IL 2 protein can be expressed in vJ38gag IL 2
