COEXISTENCE OF ADRENOCORTICOTROPIN AND SECRETOGRANIN ⅡIN THE RAT PITUITARY GLAND AFTER BRAIN INJURY
- VernacularTitle:分泌颗粒素Ⅱ和促肾上腺皮质激素在脑损伤大鼠垂体腺细胞共存
- Author:
Chunmei WANG
;
Xiaofeng HUANG
;
Jiaji YANG
;
Mei HUANG
;
Xiangdang LI
;
Dan CHEN
- Publication Type:Journal Article
- Keywords:
ACTH;
secretogranin Ⅱ;
fluorescent antibody technique;
pituitary gland;
rat
- From:
Chinese Journal of Neuroanatomy
2000;16(4):337-340,55
- CountryChina
- Language:Chinese
-
Abstract:
The localization of adrenocorticotropin(ACTH),secretogranin Ⅱ in rat pituitary gland Was studied by using specific antisera in conjunction with double immunofluorescence staining techniques.Stainings with secretogranin Ⅱ serum showed intense cytoplasmic reaction in the anterior lobe of the pituitary gland.Double immunofluorescence staining was used to identify the cells that expressed secretogranin Ⅱ and ACTH.Confocal laser scanning microscopy revealed that ACTH existed simultaneouslywith secretogranin Ⅱ in quite a number of cells.The results indicate that secretogranin Ⅱ is expressed in corticotrophs of rat pituitary gland,its physiological role may be associated with the regulation of optimal pH in the secretory vesicles for the cleavage of ACTH from its precursor.
