The inactivation of duck hepatitis virus B in blood cell suspenion induced by specific photosensitizer
- VernacularTitle:特异光敏效应对血细胞悬液中DHBV的灭活作用
- Author:
Hongyan XIONG
;
Huisun CHEN
;
Xiaobing HU
- Publication Type:Journal Article
- Keywords:
sensitizer,duck hepatitis virus B(DHBV),inactivation
- From:
Chinese Journal of Blood Transfusion
1988;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the specific photochemical effects of a newly designed target photosensitizer. Methods Based on the technique of antisense nucleic acid and the principle of photochemical reaction effects,a specific sensitizer,TFO P has been designed and synthesized.When in coordination with long wave ultraviolet ray(UVA) ,this decorated complex (TFO P) was added into the blood cell suspension to inactivate the contaminating virus( duck hepatitis virus B,DHBV).The efficacy of specific binding to DHBV DNA and viral inactivation by TFO P was detected by gel shift blot assay and infection of primary culture of duck hepatocyte.Results The designed TFO P could specifically bind to different DHBV DNA line sample and present different linking level.With a TFO P concentration of 0.1 nmol/ml and UVA intensity of 1800 ?W/cm 2,the DHBV in blood cell suspension could be reduced by 1.90～5.40 logs.Conclcusion The photochemical effects of TFO P could significant inactivate DHBV in blood.
