Inhibitory effect of laminarin (acidic polysaccharide) J201A on the proliferation of Human Lung Fibroblasts and its related mechanism of action
- VernacularTitle:海带酸性聚糖J201A抑制肺成纤维细胞增殖活性的探讨
- Author:
Benchun MIAO
;
Handong JIAN
- Publication Type:Journal Article
- Keywords:
laminarin (acidic polysaccharide) J201A;
human lung fibroblasts (HLF);
cell cy-cle;
protein synthesis;
receptors
- From:
Chinese Journal of Marine Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the inhibitory effects of laminarin (acidic polysaccharide) J201A on the proliferation of human lung fibroblasts (HLF) and its related mechanism of action. Methods: Effect of J201A on the proliferation of HLF was evaluated by MTT assay, and the effects on cell cycle and synthesis of proteins of HLF were assessed by flow cytometry. The existence of J201A receptors on HLF was confirmed by fluorescent staining assay. Results:J201 A inhibited the synthesis of proteins and the proliferation of HLF at G0/G1 stage of cell cycle and J201A receptors existed on HLF. Conclusion:J201A exerted its antifibrotic activity by inhibiting HLF at G0/G1 stage of cell cycle and the synthesis of proteins.
