Expression and Purification of Recombinant Human Endostatin
- VernacularTitle:重组人内皮细胞生成抑制因子-Endostation的表达和纯化
- Author:
Zhongyi LI
;
Junyuan WANG
;
Jiangqiu LIU
;
Linsheng CHEN
;
Ning LI
- Publication Type:Journal Article
- Keywords:
rh-Endostatin;
gene expression;
isolation;
purification
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To highly express rh-Endostatin from Pichia pastoris and purify it to homogeneity. Methods: Constructed Pichia pastoris X33/pLW202 was amplified and inoculated to ferment media. The supernatant of the strain was collected after induction. Through ultrafiltration, affinity and gel chromatography, rh-Endostatin with high purication was obtained. Results: 60mg/L rh-Endostatin was obstained from supernatant. HPLC showed its purity was above 98%. Conclusion: High level expression of secreted rh-Endostatin has been successfully achieved in Pichia pastoris expression system.
