Expression of neuropeptide Y and vasoactive intestine peptide in developmental bone and its relationship with angiogenesis and osteoblast differentiation
- VernacularTitle:神经肽Y和血管活性肠肽在发育骨中的表达
- Author:
Yu QIN
;
Guoxian PEI
- Publication Type:Journal Article
- Keywords:
Neuropeptide;
Vasoactive intestine peptide;
Bone development;
Angiogenesis;
Osteoblast;
Differentiation
- From:
Chinese Journal of Orthopaedic Trauma
2002;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression patterns of neuropeptide Y(NPY)and vasoactive intestine peptide(VIP)during the bone development,and its possible relationship with angioge nesis and osteoblast differ-entiation.Methods The bones of low extremity of SD embry o and new -born rats were taken,fixed in formalin and packed by paraffin wax.The developmental stages of bone were determi ned by HE stain The representative specimen were picked out and within t he same layer the sections were taken immunohistochemical stain respective to anti-NPY,VIP,vasoepithelial grow th factor(VEGF)and alkaline phosphatatase(AP).The location and intensity of stain were compared by microscopi c observation.Results During the earliest stage of osteoge nesis,there was no sign of angiogenesis while osteob last began to differentiate and it parallels the intensity of ossificati on activity.At the first ossification center,the l ocation and pattern of NPY expressio n are similar to those of VEGF.Conclusions Due to VIP expression at the earliest stage of osteogenesis,the bone tissue may have the independent nerve control before angiogenesis.The expression similarity of timing and lo cation between NPY and VEGF suggests there is certain relationship between NPY-energetic nerve and angiogenesis.
