Study on the mithridatism of VMAT_2 in transgeneic CHO cell
- VernacularTitle:转基因CHO细胞中VMAT_2抗毒性作用的研究
- Author:
Min YE
;
Xinsheng DING
;
Hairong DONG
- Publication Type:Journal Article
- Keywords:
cDNACHO VMAT 2 Mithridatism MPP + Reserpine
- From:
Journal of Clinical Neurology
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mithridatism of VMAT 2 in transgeneic CHO cell.Methods Using technology of transgene from PC 12 to CHO, MTT reduction assay was used to detect the toxic effect on MPP + to wtCHO and cDNACHO,meanwhile the role of reserpine was observed,including the toxic effect to MPP + on specific blocking agent of VMAT 2.Results cDNACHO to the sensitivity of MPP + was much less than that of wtCHO over concentration of 0.5 mmol/L MPP +; cDNACHO had the same sensitive as wtCHO to rotenon;after the reserpine was added,the above role disappeared,but wtCHO reserpine was given alone,it couldn't change its sensitivity to MPP +.Conclusion VMAT 2 has protective effect on cDNACHO by transporting MPP + to vesicles; PC 12 possesses the antitoxic components.
