Resistance mechanism of erythromycin in Streptococcus pneumoniae
- VernacularTitle:肺炎链球菌对红霉素耐药机制的研究
- Author:
Hui WANG
;
Minjun CHEN
- Publication Type:Journal Article
- Keywords:
Streptococcus, pneumoniae;
Erythromycin;
Serotyping;
Genes, erm/mef
- From:
Chinese Journal of Laboratory Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate resistance mechanism against erythromycin in Streptococcus pneumoniae from Beijing region. Methods 116 strains of erythromycin resistant Streptococcus pneumoniae were collected from 1998 to 1999 at Peking Union Medical College Hospital Serotyping was done with "capsular swelling" technique erm/mef genes were detected with PCR, pulsed field gel electrophoresis (PFGE) and penicillin binding protein (PBP) fingerprinting technique were used to detect the DNA of resistant strains Results The prevalent serotypes in the 116 erythromycin resistant strains were 23F(30 0%),6A(19 0%),19F(13 8%),15(7 8%),23A(5 2%) 95 7% of penicillin resistant strains were also macrolide resistant, most (85%) expressing the MLS phenotype with co resistance to clindamycin The macrolide resistance determinant in 86 4% of erythromycin resistant strains was the erm gene, both the erm and mef genes were found in 6%, mef alone in 1 7% and no mechanism in 4 2% PFGE identified two clones: one a serotype 23F clone resistant to penicillin; and the other a penicillin susceptible and macrolide resistant serotype 6A clone Conclusions Ribosomal modification (erm gene coded) was the main resistance mechanism against erythromycin in Streptococcus pneumoniae in Beijing region Two resistance clones bear concern
