Telomere dynamics of leukocytes in bone marrow with leukemia and myelodysplastic syndromes by flow cytometry
- VernacularTitle:流式原位杂交法检测白血病和骨髓异常增生综合征患者端粒长度的研究
- Author:
Wei CUI
;
Jianmeng CHEN
- Publication Type:Journal Article
- Keywords:
Flow cytometry;
In situ hybridization, fluorescence;
Telomere;
Leukemia;
Myelodysplastic syndromes
- From:
Chinese Journal of Laboratory Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study telomere dynamics in bone marrow from patients with leukemia and myelodysplastic syndromes (MDS) by flow cytometry and FISH (FLOW FISH) Methods By FLOW FISH , we detected telomere lengths of leukocytes in bone marrow from 43 leukemia patients and 48 healthy donors The mean fluorescence intensity of cells (Q FISH) was detected by flow cytometer Results Compared with the age matched normal range (0 066?0 015), 38 of 43 patients had shortened telomere length (0 047?0 019), and the remaining patients (3 MDS and 2 chromic phase CML) had normal telomere lengths (0 069?0 007 vs 0 060?0 008) Although no significant difference in telomere lengths could be demonstrated in 8 leukemia patients at diagnosis and follow up, a trend toward telomere shortening was observed in CLL patients with 0 052 vs 0 048 in which telomere tended to shorten as the disease went on In contrast, longer telomeres were found in AML patients after induction chemotherapy (0 056) compared with those found in diagnostic specimens (0 043) In the healthy donors, the telomere length turned to be shorter with increase of age ( r =0 67), whereas in leukemia and MDS patients, the telomere length had no correlation with age ( r =0 14) Conclusion The telomere lengths from most patients with leukemia and MDS were shorter than that of healthy donors The telomere dynamics is associated with prognosis and therapy of disease
