Detection of cdc6 gene in exfoliate cell in urine
- VernacularTitle:尿脱落细胞中细胞分裂周期蛋白6基因的检测
- Author:
Cheng AN
;
Yan BAI
;
Xiumin ZHANG
;
Zhenhua YANG
;
Man ZHANG
- Publication Type:Journal Article
- Keywords:
Bladder neoplasma;
Genes, cell division cycle;
Reverse trascriptase polymerase chain reaction;
Gene expression
- From:
Chinese Journal of Laboratory Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective DNA replication increases during tumor cells limitlessly proliferate. To develop a method that can early diagnose and routine screen out bladder cancer, but it does not impair patients by detecting cdc6 in exfoliate cell in urine of patients with bladder cancer. Methods 200 ml fresh urine of patients with bladder cancer or non bladder cancer who voided urine after morning was collected and centrifugated for urine sediment, then total RNA was extracted with TRIZOL reagent from it and detected the expression of cdc6 gene by RT PCR. Results 28 samples were positive and 2 samples were negative in 30samples of bladder cancer, positive rate was 93.3%. Positive rate was 16.7% in 30 samples of non bladder cancer. Compared with the sensitivity of urine cytology, that of cdc6 RT PCR increases markedly ( P
